Tth ssb
WebJan 24, 2013 · The four SSB proteins were divided into two groups based on primary sequence analysis. tth and bl21 were similar to that of E. coli (NCBI LOCUS: 1EYG_D), … WebAfter binding single-stranded DNA, SSB destabilizes helical duplexes, thereby allowing DNA polymerases to access their substrate more easily. SSB is involved in DNA replication and …
Tth ssb
Did you know?
WebApr 13, 2024 · This map is for visual representation only and is not to be used for navigation or enforcement. Roseway Basin – Area to be Avoided. The Roseway Basin, located … WebJun 21, 2006 · To study the biochemical properties of single-stranded DNA-binding (SSB) protein from Deinococcus geothermalis (DgeSSB), we have cloned the ssb gene obtained by PCR and developed an overexpression system. The gene consists of an open reading frame of 900 nucleotides encoding a protein of 300 amino acids with a calculated molecular …
WebJan 1, 2006 · The Tth SSB-255 mutant protein was also purified using the same strategy (data not shown). The final products were shown to be 99% pure. Properties of Tth SSB … WebSSB is known to bind ssDNA, protecting it from cleavage by endonucleases. 4 Furthermore, SSB has been found to reduce secondary structure of ssDNA, improve PCR specificity, …
WebDec 25, 2013 · NaCl, followed by a buffer wash to remove unbound Tth SSB proteins. The buffer containing the indicated Tth SSB and NaCl concentration was then. injected into … WebApr 6, 2024 · On April 11, 2024 at 15:47:36 ET an unusually large $175.00K block of Call contracts in South State (SSB) was sold, with a strike price of $70.00 / share, expiring in …
WebIncluding ET SSB in TtAgo reactions can improve cleavage of higher GC content substrates In some cases the addition of ET SSB ( NEB #M2401 ) can aid in the cleavage of higher …
WebOct 1, 2002 · The expression was induced by 0.5 mM IPTG, but it was accompanied by strong inhibition of culture growth.The best induction of thermostable SSB-like proteins … list of spice girlsWebThis “step up” feature of the SSB reflects the objective of the Savings Bond programme, which is to facilitate long-term savings and investment. Coupon rates for each issuance … Please note that you will not be able to amend or cancel submitted redemption … list of spices and seasoningsWebJan 1, 2005 · Abstract. In this paper we report that the inclusion of heat-resistant RecA protein from a thermophilic bacteria, Thermus thermophilus, and its cofactor (ATP) in PCR effectively eliminates non-specific PCR products.The effect of RecA protein, which catalyzes pairing between homologous DNA molecules with great fidelity in genetic recombination, … list of speyside scotch brandsWebApr 5, 2008 · The thermophilic bacterium Thermoanaerobacter tengcongensis has two single-stranded DNA-binding (SSB) proteins, designated TteSSB2 and TteSSB3. In a SSB complementation assay in Escherichia coli, only TteSSB3 took over the in vivo function of EcoSSB. We have cloned the ssb genes obtained by PCR and have developed E. coli … immersion refractometerWebAug 15, 2009 · T. thermophilus SSB (TthSSB)–F255P and wild-type TthSSB proteins were prepared as described previously [8]. The proteins were shown to be 99% pure. The … immersion refers to:WebssDNA Binding Proteins. Product Listing Product Overview. NEB offers highly thermostable ssDNA binding proteins that are ideal for nucleic acid amplification and sequencing (Tth … list of spiciest pepperWebTtAgo is a prokaryotic argonaute from the Gram-negative, thermophilic bacterium Thermus thermophilus which functions as a DNA-guided endonuclease when provided with a 16–18 nucleotide long 5′-phosphorylated single-stranded DNA oligonucleotide guide. The RNase H-like active site of TtAgo requires divalent Mg 2+ (or Mn 2+) (See product usage note #1) … immersion reflection essay