Topvision agarose
WebThermo Scientific TopVision Agarose provides optimal concentration between 0.7 to 2% in all typical buffer systems. It is a highly purified agarose with very low EEO values certified … Thermo Scientific TopVision Agarose provides optimal concentration between … Thermo Scientific TopVision Agarose provides optimal concentration between … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebTopVision Agarose Tablets is a low-electro endosmosis, multi-purpose agarose in tablet form, delivered in a convenient blister pack. It is made of standard melting point agarose, …
Topvision agarose
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WebThe Thermo Scientific TopVision Agarose Tablets, low EEO (LE), are a multi-purpose agarose in tablet format delivered in a convenient blister pack. The agarose tablets are … Web• TopVision Agarose Tablets (Cat no. R2801) • GeneRuler 1 kb DNA Ladder (Cat.no. SM0311) • 0.2 or 0.5-mL nuclease-free microcentrifuge tubes • Water, nuclease-free. Perform PCR. 1. Prepare reaction by adding the following components in the order listed in the following table. Component 20 µL rxn 50 µL rxn Final conc. 5X Phusion ...
WebOct 17, 2024 · An 8-μl aliquot of amplified PCR product was separated by gel electrophoresis on 1.5% (w/v) agarose gels (TopVision agarose; Thermo Fisher Scientific Inc.) in 1 × AccuGENE TAE buffer (Lonza, Verviers, Belgium) for 4 h at 120 V, stained with GelRed™ Nucleic Acid Gel Stain (Biotium Inc., Hayward, CA, USA), and photographed under UV ... Web2. Prepare 1% TopVision™ Agarose gel as follows: • stir 1g of agarose powder in 72 ml of deionized water; • melt the agarose, and then add 10 ml of 10X MOPS buffer and mix; • when the agarose solution cools to 60°C, add 18 ml of fresh formaldehyde (37%) in a fume hood and mix thoroughly; • pour the gel. 3.
WebTopVision Agarose Tablets, low EEO (LE), is multipurpose agarose in tablet format delivered in a convenient blister pack. The agarose tablets are made of standard melting point agarose that yields high resolution, sharp DNA bands with high clarity and low background. WebOct 31, 2024 · PCR products were electrophoresed in 2% agarose gel (TopVision Agarose Tablets R2801; Thermo Fisher), and bands were excised and extracted using QIAQuick Gel Extraction kit (catalog number 28706; Qiagen). GeneWiz performed the Sanger sequencing. Software used for design and analysis of mutant cell lines include CRISPRdirect, …
WebThermo Scientific TopVision Agarose is highly purified agarose with very low EEO values certified by strict quality control test procedures. Characteristics Electroendosmosis EEO, -M r 0.08-0.11 Gel strength (1% gel) 1200 g/cm2 Gel strength (1.5% gel) 2300 g/cm2 Gel point (1.5% gel) 34.0-38.0°C ...
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