Thioredoxin fusion expression vector
WebAbstract. This unit describes a gene fusion expression system that uses thioredoxin, the product of the Escherichia coli trxA gene, as the fusion partner. The inherent thermal … WebRecombinant Production of Crab Antimicrobial Protein Scygonadin Expressed as Thioredoxin and SUMO Fusions in Escherichia coli. Appl Biochem Biotechnol.. 2013-03; 169(6):1847-1857 Li Y. Protein Production Core Facility, Department of Biochemistry, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.
Thioredoxin fusion expression vector
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WebWe have developed a versatile Escherichia coli expression system based on the use of E. coli thioredoxin (trxA) as a gene fusion partner. The broad utility of the system is illustrated by the production of a variety of mammalian cytokines and growth factors as thioredoxin fusion proteins. Although m … WebThis 1.4 kb fragment was cloned into the high-expression vector pET32a, under control of the T7 promoter. Expression of this plasmid in Escherichia coli BL21(DE3) resulted in the production of a fusion protein ( approximately 62 kDa) consisting of 112 amino acids of thioredoxin and approximately 450 amino acids of fragment C.
WebThis plasmid is an empty vector to be used with a LIC cloning protocol.It has a TEV cleavable His6-Thioredoxin tag on the N-terminus and Amp resistance. To clone into this … pET His6 Thioredoxin TEV expression vector with BioBrick polypromoter … WebMay 1, 1997 · This unit describes a gene fusion expression system that uses thioredoxin, the product of the Escherichia coli trxA gene, as the fusion partner. The inherent thermal …
WebThe pET-32 vector series is designed for high-level expression of recombinant thioredoxin fusion proteins. Previous studies have shown that linkage to thioredoxin increases the … WebDec 27, 2024 · Human enterokinase light chain (hEKL) cDNA sequence was designed with the help of codon optimization towards Escherichia coli codon preference and ribosome binding site design and artificially synthesized with a thioredoxin fusion tag at the N-terminal and a five his-tag peptide at the C-terminal. The synthetic hEKL gene was cloned into the …
WebFeb 1, 1993 · We have developed a versatile Escherichia coli expression system based on the use of E. coli thioredoxin (trxA) as a gene fusion partner. The broad utility of the system is illustrated by the ...
WebAbaecin, a 34-aa-long antimicrobial peptide from honeybees, was expressed in a fusion form to 6xHis-SUMO in a new vector system to evaluate the prokaryotic expression platform of the antimicrobial peptides. The fusion sequences were codon-optimized in three different combinations and expressed in E. coli. the last call coWebThe pET-32 vector series is designed for high-level expression of recombinant thioredoxin fusion proteins. Previous studies have shown that linkage to thioredoxin increases the yield of biologically active proteins expressed in Escherichia coli. How thioredoxin promotes protein solubility is not known, although a catalytic contribution thymegoryaWebJul 12, 2010 · These NK1 mutants were made with the additional R134G mutation to aid in thioredoxin tag removal and purification . ... was expressed as a 6xHis-thioredoxin fusion protein from the expression vector pET-Duet1 in the Escherichia coli strain Rosetta/gami(DE) (Novagen) to promote disulfide bond formation. The biotinylated … the last byzantine emperor wasWebThe pET-32a (+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein (1). Cloning sites are … the last call britboxWebMar 1, 2009 · To explore a new approach for high expression of the G13 domain, we fused the sequence encoding G13 to thioredoxin (Trx) gene to construct the recombinant expression vector (pThioHisA-G13). thyme golden variegatedWebMar 1, 1993 · The pilot expression resulted in the production of the putative 62 kDa AlkM-thioredoxin and the 50 kDa CatA-thioredoxin fusion proteins, and the identity of the expressed proteins was verified to ... thyme good for diabetic 2WebFeb 1, 1993 · Abstract. We have developed a versatile Escherichia coli expression system based on the use of E. coli thioredoxin (trxA) as a gene fusion partner. The broad utility of the system is illustrated by the production of a variety of mammalian cytokines and growth factors as thioredoxin fusion proteins. Although many of these cytokines previously ... thyme gorya