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Pslf172

WebFeb 22, 2002 · All pSLF172 constructs express proteins in-frame with three C-terminal hemagglutinin (HA) epitope tags, which were detected by immunoblotting with the 12CA5 anti-HA monoclonal antibody (mAb). An insert containing ark1 + ::HA3 and the nmt1 terminator was subcloned from pSLF172- ark1 + into pJK210 using Xho I and Sac I to … WebDec 30, 2005 · This restriction product was ligated into Bgl II/ Sal I-digested pSLF172 together with Nco I/ Xho I digested PCR product generated with oligonucleotides A and C (see above). The pnmt-GFP and pcpy-GFP vectors have been described elsewhere [25]. Protein extraction and Western analysis.

Meiotic recombination cold spots in chromosomal cohesion sites

WebPlasmid pREP3‐adh: The nmt1‐MCS‐3xHA‐term cassette from pSLF172 (Forsburg and Sherman, 1997) was cloned as a PstI/SacI fragment into pREP3X (gift of S.L. Forsburg), giving rise to plasmid pREP3X‐tag. Subsequently a XhoI/NotI fragment from pREP3X‐tag was substituted with a hybridized tw75/tw76 primer adapter giving pREP3‐SX. WebPurification of Hsk1, a Minichromosome Maintenance Protein Kinase from Fission Yeast* (Received for publication, February 6, 1998, and in revised form, June 16, 1998) au メール 構成プロファイル https://orchestre-ou-balcon.com

The Fission Yeast Homologue of Orc4p Binds to Replication …

WebOct 1, 2024 · 4 beds, 2 baths, 1624 sq. ft. house located at 172 Longfellow Dr, Palm Springs, FL 33461 sold for $370,000 on Oct 1, 2024. MLS# RX-10646249. Modern, sleek, European … WebThe S. pombe expression vector pREP3X, pSLF172, and pSLF173 series were obtained from recombinant DNA vec-tors in the American Type Culture Collection (ATCC) (Table 1). To construct fluorescent protein-tagged vectors, the coding sequences of the fluorescent proteins were cloned into the S. pombe autonomously replicating sequence (ARS) element- WebpSLF172,272,372 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially … auメール 機種変更 引き継ぎ

The Schizosaccharomyces pombe Aurora–related Kinase …

Category:Multiple Domains of Fission Yeast Cdc19p (MCM2) Are Required …

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Pslf172

Protein O‐mannosylation is crucial for cell wall integrity, septation ...

WebJun 3, 1997 · As shown in Fig. 2 A, an anti-Cdc19p antibody recognizes a low amount of endogenous Cdc19p protein in the control lysate, and substantially increased amounts in … WebNov 2, 2024 · Groundswell Art Showcase. 4:15 - 5:00 PM in the Art Room. P.S. 172 Beacon School of Excellence. Mar 31.

Pslf172

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WebMar 17, 2014 · 2003). 3HA tag was fused in frame to the C‐terminus of fission yeast Rec8 protein using pSLF172 (Forsburg & Sherman 1997). All strains used in this study are listed in Table S3 (Supporting Information). Synchronous meiotic cultures of budding and fission yeast and monitoring meiotic progression were conducted as described previously (Ohta … Webof pSLF172 (38). The orp41 gene was synthesized by PCR and inserted into the NotI site of pRCE3X. Strain YRC4 (h2 ura4-D18 pRCE3X-orp4N), expressing the N-terminal half of Orp4 protein (amino acids 1–486), and strain YRC5 (h2 ura4-D18 pRCE3X-orp4C), expressing the C-terminal terminal half of Orp4 protein (amino acids 436–972), were constructed

WebMay 10, 2002 · The plasmid pRCE81X was derived from the plasmid pREP81X (30) by replacing the 2.2-kb nmt1 promoter-terminator DNA fragment with thenmt1 promoter-terminator DNA fragment of pSLF172 (31). Theorc4 + gene was synthesized by PCR and inserted into the XhoI/NotI sites of pRCE81X, yielding pRCE81X-orc4. WebMar 16, 1999 · The origin recognition complex (ORC) was originally identified in the yeast Saccharomyces cerevisiae as a protein that specifically binds to origins of DNA replication. Although ORC appears to play... The origin recognition complex (ORC) was originally identified in the yeast Saccharomyces

WebOct 28, 2005 · Next, GFP was isolated as an NcoI–NotI fragment from pcpy-GFP and ligated together with XhoI/NcoI digested PCR product and XhoI/NotI digested pSLF172, … WebELLULAR DNA replication requires precise regu- lation to ensure that the genome is replicated once completely in each cell cycle, and that the process of replication is coordinated with proper cell cycle entry and subsequent nuclear division.

WebJun 10, 1997 · The source of Cig2 kinase, GBY228, is a strain carrying a plasmid expressing the cig2 + ORF fused at its C terminus to a triple hemaglutinin (HA) tag from the nmt1 …

Webepitope in its C-terminal region. To construct pSLF172(L)-pka1, the pka1 gene from pSLF372(L)-pka1 was digested with BglII and NotI. The digested fragment was cloned … au メール 添付ファイルWebThe vector was constructed by cutting pSLF172 with SacI and XhoI, isolating the fragment containing the HA polylinker, and ligating into XhoI-SacI digested REP42X. Mycoplasma … au メール 添付ファイル 保存先WebJun 10, 2005 · The synthesis of O-mannosyl glycans is initiated in the endoplasmic reticulum (ER) by the transfer of a mannosyl residue from dolichyl phosphate-activated mannose (Dol-P-Man) to specific serine/threonine residues of proteins entering the secretory pathway ( Strahl-Bolsinger et al ., 1999; Willer et al ., 2003 ). au メール 添付ファイル 受信できないWebpSLF172 87609 ™ Clone type Vector Applications Molecular biology Product format Freeze-dried See Additional Product Information Buy Now Price: $590.00 EA Discounts may be … au メール番号 m15WebPcb-Power Supply; Led Tv Pd Bd, Pd46Cf2N_Zsm, Pslf172. Brand New. Available as a Special Order - Normal delay is 4 to 5 business days + shipping days. Order Now $ 253.98. Preorder. Qty. Questions ? Ask us About This Product. More Details; Warranty; COMPATIBLE MODELS. Samsung UA46C7000WRMXL. 90 Day Factory Warranty ... au メール 添付ファイル 容量 増やすau メール 無料ゲームWebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies. au メール 確認コード 届かない