WebFeb 22, 2002 · All pSLF172 constructs express proteins in-frame with three C-terminal hemagglutinin (HA) epitope tags, which were detected by immunoblotting with the 12CA5 anti-HA monoclonal antibody (mAb). An insert containing ark1 + ::HA3 and the nmt1 terminator was subcloned from pSLF172- ark1 + into pJK210 using Xho I and Sac I to … WebDec 30, 2005 · This restriction product was ligated into Bgl II/ Sal I-digested pSLF172 together with Nco I/ Xho I digested PCR product generated with oligonucleotides A and C (see above). The pnmt-GFP and pcpy-GFP vectors have been described elsewhere [25]. Protein extraction and Western analysis.
Meiotic recombination cold spots in chromosomal cohesion sites
WebPlasmid pREP3‐adh: The nmt1‐MCS‐3xHA‐term cassette from pSLF172 (Forsburg and Sherman, 1997) was cloned as a PstI/SacI fragment into pREP3X (gift of S.L. Forsburg), giving rise to plasmid pREP3X‐tag. Subsequently a XhoI/NotI fragment from pREP3X‐tag was substituted with a hybridized tw75/tw76 primer adapter giving pREP3‐SX. WebPurification of Hsk1, a Minichromosome Maintenance Protein Kinase from Fission Yeast* (Received for publication, February 6, 1998, and in revised form, June 16, 1998) au メール 構成プロファイル
The Fission Yeast Homologue of Orc4p Binds to Replication …
WebOct 1, 2024 · 4 beds, 2 baths, 1624 sq. ft. house located at 172 Longfellow Dr, Palm Springs, FL 33461 sold for $370,000 on Oct 1, 2024. MLS# RX-10646249. Modern, sleek, European … WebThe S. pombe expression vector pREP3X, pSLF172, and pSLF173 series were obtained from recombinant DNA vec-tors in the American Type Culture Collection (ATCC) (Table 1). To construct fluorescent protein-tagged vectors, the coding sequences of the fluorescent proteins were cloned into the S. pombe autonomously replicating sequence (ARS) element- WebpSLF172,272,372 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially … auメール 機種変更 引き継ぎ