Hbad-mcherry-egfp-lc3
A detailed list of materials is provided in Table 6. Important: All hits in screening approaches have to be verified using orthogonal methods to assess autophagy (for a comprehensive … See more A detailed list of materials is provided in Table 5. 1. 2.1. Grow cells in either F-bottom (adherent cell types) or V-bottom (suspension cell types) 96-well plates. Seeding of 50,000 cells per well for adherent cells and … See more For the construction of the cell lines use low passage numbers. For accurate autophagy measurements, make sure that the cells never overgrew, even during the construction of the … See more WebTo get more information about a location, hover your mouse over the location.
Hbad-mcherry-egfp-lc3
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Webtandem mCherry-GFP-LC3 construct in neurons through the nervous system and demonstrated efficient and accurate measurements of LC3 flux after pharmacological … WebDec 27, 2024 · LC3(light chain 3)简称于MAP1LC3 (microtubule-associated proteins light chain 3),是目前公认的自噬标记物。哺乳动物中的LC3与酵母中的自噬相关蛋 …
WebWe detail here a protocol using tandem-tagged mCherry-EGFP-LC3 (C-G-LC3) to quantify autophagic flux in single cells by ratiometric flow cytometry and to isolate subpopulations … WebMCF7 cells stably expressing the fusion protein enhanced green fluorescent protein (eGFP)–LC3 (MCF7 eGFP-LC3 cells) were used to investigate the LC3 response upon ablation laser–induced plasma membrane injury.
WebThe mouse was infected with mCherry-EGFP-LC3 adeno-associated virus (100 μL) via the tail vein four weeks before sacrifice. The liver was removed carefully and trimmed immediately after the mouse was sacrificed. Then, the tissue was embedded in optimal cutting temperature (OCT) compound (Sakura, Torrance, USA), serial sections (6 μm) … WebFeb 19, 2024 · Flow cytometry software like Flow Jo can run a ratiometric calculation as a derived parameter, where autophagic flux is the mCherry signal divided by the GFP signal. This assay is simple and quick with a 1-2 hr process time after treatment and is the most quantitative way to measure autophagic flux. Request Your Autophagy Handbook Today.
WebTandem fluorescent-tagged LC3 (mRFP-EGFP-LC3) is a convenient assay for monitoring autophagic flux based on different pH stability of EGFP and mRFP fluorescent proteins. …
WebFeb 3, 2024 · Cherry Barbs are long, thin, brightly colored fish. As the name suggests, they are bright red, with the male species generally being more vibrant than the females, … checkpoint woods tarkovWebC3003-100μl. Lenti-EGFP-LC3B (10^9TU/ml) 100μl. 2992.00元. Lenti-EGFP-LC3B,即Lentivirus expressing EGFP-LC3B fusion protein,是一种可以表达EGFP-LC3B融合蛋白的慢病毒,可以用于感染细胞或组织后进行细胞自噬 (autophagy)检测。. 本产品带有嘌呤霉素 (puromycin)抗性,感染细胞后可以筛选 ... flat military headstoneWebThe whole mCherry-GFP-LC3 expression cassette was excised from a plasmid kindly provided by Terje Johansen's laboratory, and inserted into a shuttle proviral plasmid, containing both the AAV2/2 inverted terminal repeats and the 1.6-kb cytomegalovirus enhancer promoter (Fig. 1 A). checkpoint workhuman.comWebFeb 8, 2024 · The mCherry-EGFP-LC3 (ptfLC3) plasmid was a gift from Tamotsu Yoshimori (Addgene, 21074; deposited by Yoshimori lab), which shows yellow puncta (due to colocalization of EGFP and mCherry) upon autophagosome formation, while red puncta (due to quenching of EGFP in lysosomal acidic pH) is observed upon formation of … checkpoint worldboxWebNov 7, 2015 · Both clones expressed 75kDa mCherry-EGFP-LC3 fusion protein, while clone relativelylow expression level (Fig. 1C). faintband 25kDa detected westernblots may represent free EGFP protein. freeEGFP synthesizeddur- ing translation because EGFPstart stopcodons morelikely producedfrom lysosomal degradation tfLC3B(Hosokawa et al., … checkpoint_wrapperWebSearch for Atlanta luxury homes with the Sotheby’s International Realty network, your premier resource for Atlanta homes. We have 2,082 luxury homes for sale in Atlanta, and … checkpoint wordWebTransgenic GFP-LC3 mice are a valuable tool to investigate autophagy ; however, comparison of homeostatic and disease responses between GFP-LC3 and wild-type (WT) mice has not been done. We examined the effects of GFP-LC3 expression on autophagy, acinar cell function, and experimental pancreatitis. flat military style boots